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Next, proteins were treated with 3C-HRV protease (Genescript, Z03092) overnight at 4 C without shaking according to the manufacturers instructions

Since the valinomycin synthetase Vlm shows the same genetic architecture as Ces, it is tempting to speculate that the novel mechanism of non-ribosomal peptide assembly proposed in this study is not a specificity of the cereulide synthetase but a more common logic for biosynthesis of ester bond containing NRPS products, thereby contributing to biodiversity of natural depsipeptide products

Alterations in the microbiome can impact the expression of tight junctions [154], potentially compromising these barriers to microorganisms and inducing neuroinflammation [149]

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