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Inclusion criteria: all participants must: be 65 years of age be attending a primary healthcare centre for consultation on some medical matter provide their informed consent to be included have a serum B12 concentration of 200 pg/ml at 4 months in over 70% of patients

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In fact, it has been demonstrated that caspase-3 leads to ZO-1 and claudin-5 disruption in endothelial cells even in the absence of apoptotic death, indicating a non-apoptotic role in barrier regulation (Zehendner et al., 2011