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Six weeks aligns with animal study timelines showing measurable tissue healing and represents a reasonable decision point
The intracellular concentration of gamma-glutamylcysteine (GGC) is extremely low (~ 5 uM) when GS is present, thus GCL is considered the rate-limiting enzyme [6]
Fecal samples were collected from these human study participants in the sterile fecal containers, and stored in a 80 C freezer until BA analysis
The overnight cultures were used as seed cultures for 1 L of fresh LB medium containing 50 g ml 1 kanamycin and incubated at 37 C until the OD 600 reached 0.8
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