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Sampling should be geographically stratified and included isolates from sesame and key rotation crops, with accompanying metadata on temperature regime, rainfall/aridity, soil properties, irrigation practices, and cropping history, to assess host-associated structure, genotypeenvironment relationships, and gene flow (Burkhardt et al

The femur-graft-tibia complexes were vertically aligned, and tensile tests were performed at a crosshead speed of 40 mm/min with a 50N load cell until graft failure was detected by an abrupt drop in loading

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