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For precise dosing within research protocols: 1 ml = 100 units (IU) = 100 clicks 0.5 ml (one full rotation of the mechanism) = 50 clicks Examples of dosing for research purposes: 1 click = 0.05 mg (50 mcg) of active substance 2 clicks = 0.10 mg (100 mcg) of active substance 4 clicks = 0.20 mg (200 mcg) of active substance 10 clicks (0.1 ml) = 0.50 mg (500 mcg) of active substance 20 clicks (0.2 ml) = 1.00 mg (1000 mcg) of active substance 50 clicks (0.5 ml / full rotation) = 2.50 mg (2500 mcg) of active substance This mechanical system ensures absolute precision, repeatability, and flexibility when setting micro-doses according to the needs of a specific laboratory protocol, without the need for complex manual dilution

Inclusion criteria consisted of: (1) aged between 18 and 75 years