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The reaction time was defined by placing the mouse on the hot plate, and the time was recorded when the animal showed hind paw licking or jumping, and the 45 sec period was observed to avoid tissue damage

doi: 10.21037/atm.2018.07.32

If, as one post-mortem studies suggest [81], glutamate concentrations in different cortical layers can vary in opposite direction in schizophrenia, the restricted spatial resolution is an even more critical limitation (even the available 1 mm3 resolution of most other MRI techniques cannot yet address this hurdle)

You can read the full article at In order to assess the safety and effectiveness of tirzepatide as an additional treatment for Japanese patients with type 2 diabetes, a phase 3 trial was conducted