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To model food restriction, the authors used semaglutide, a glucagon-like peptide-1 receptor (GLP1R) agonist that induces fasting-like metabolic responses

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We found that especially PSPH V116I mutant cells accelerated their 2D growth under physiological (low) glucose conditions (Figure S7B, p = 0.0013 for PSPH V116I ), and both GBM isogenic models showed a 2-fold increase in clonogenic capacity in 3D alvetex assays under low glucose conditions (Fig