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Materials and methods Reagents and media used in this study NAC, ciprofloxacin, trypsin-EDTA, gentamicin, Triton X-100, resazurin, glycerol, penicillin/streptomycin solution and kanamycin were purchased from Sigma-Aldrich, Sydney, Australia

doi: 10.1371/journal.ppat.1007355 72 HardisonR

In contrast, GHK-Cu has been extensively studied since it was first discovered in the 1970s, and the benefits attained from its consistent application are well-documented

If youve reconstituted a 5mg vial with 2mL water (2.5mg/mL concentration) and want to increase from 0.5mg to 0.75mg per dose, draw 0.3mL instead of 0.2mL