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A box was constructed to fully enclose the small molecule and protein by adjusting the Grid Box parameters, followed by running AutoGrid 4, setting the docking parameters and algorithms, running AutoDock4, and exporting the results file in PDBQT format

Each experiment consisted of three independent biological replicates and each replicate was carried out at different times

GSH augmentation deserves investigation as a beneficial therapeutic approach for not only PD but also numerous other CNS disorders for which GSH deficiency and GSH-related enzyme deficits have been documented (multiple sclerosis, 25,26 autism, 2729 Alzheimers disease, 30,31 schizophrenia, 32,33 and bipolar disease 34 )

(Frontiers in Pharmacology, 2022) reports a half-life under 30 minutes after IM or IV administration in rodent and dog models, which is why split-dose schedules appear in the literature