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On day 6, cells were counted, and 100 l cell suspension (in DMEM media supplemented with only 10% FBS but no antibiotics) was added to the 96-well plates (containing siRNA and transfection mix) at the appropriate seeding density (12k for DMSO and 15k for etoposide-treated cells)
Once these levels are established, the speaker suggests eliminating one food group at a time to identify potential triggers
A single 100 mg vial contains more elemental copper than the oral Tolerable Upper Intake Level for an entire day and roughly 30 to 50 times the daily copper dose that clinicians deliver intravenously to patients on total parenteral nutrition, where copper is dosed conservatively precisely because the parenteral route bypasses gut regulation
IM suits volumes above 1 mL and absorbs faster
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