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52 As previously reported, GLP-1RAs promote antihypertensive effects by enhancing vasodilation through nitric oxide production and modulating the RAAS, reducing plasma angiotensin II levels

For example, Gros et al

Briefly, jejunum (10 mg) was homogenized in DNA lysis buffer (pH 8.0) [100 mM TrisHCl (pH 8.0), 5 mM EDTA, 0.2% SDS, 200 mM NaCl] and incubated with proteinase K solution (Sigma) at 55 C overnight