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In detail, a NanoWizard 3 Bioscience with piezo range up to 15 m (JPK Instruments, Germany) was used for the shortest cell-surface contact time and a CellHesion 200 (JPK Instruments, Germany) with 100 m piezo travel length was used for longer cell-surface contact times (5 s, 10 s) in order to make sure that the cell detachment length can fully be reached with the piezo

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MOTS-c was found to upregulate key genes in the TGF-/Smad pathway (such as TGF-1, TGF-2, and Smad7) and increase the expression of bone formation markers like alkaline phosphatase (ALP), osteocalcin, and Runx2 in bone cells