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AP-MS analysis Five grams of a mixture of 12-day-old seedlings and adult plant inflorescences was ground into powder in liquid nitrogen and then resuspended in 20 mL ice-cold lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgCl 2 , 10% glycerol, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, and 1 protease inhibitor cocktail, Roche) for 20 min at 4 C with rotation

4) the paper was a review article, conference abstract or comment on a previously published article