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Each reaction contained 500 ng DNA template, 0.4 l Cy3-UTP (5 mM) and 0.4 l RiboLock RNase Inhibitor (Thermo Fisher Scientific) and was incubated for 2 hours at 37C

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Additionally, understanding the risk factors for gallbladder disease, such as obesity and rapid weight loss, can help individuals make informed lifestyle choices to reduce their risk

Common symptoms include tiredness, lethargy, feeling faint, becoming breathless