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The trachea was cannulated, and animals were mechanically ventilated with room air using a positive pressure ventilator (Harvard rodent ventilator, tidal volume of 1 ml/100 g of body weight, ventilator frequency 60 strokes min 1 for rats, 150 strokes min 1 for mice)

Rather than introducing growth hormone directly, it gently signals your pituitary gland to produce and release more of your own, preserving the natural rhythm of how growth hormone is meant to be released across the day and during deep sleep

Nat Med (2002) 8(7):6434.10.1038/nm0702-643 80 TschopMSmileyDLHeimanML