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Recombinant TGR was subsequently expressed in the Escherichia coli strain BL21(DE3) in the presence of pSUABC [14] in LB medium supplemented with 20 M flavin adenine dinucleotide, and otherwise conditions for optimal selenoprotein expression were followed as described [15]

Future Research Priorities in Safety and Tolerability Despite the promising role of GLP-1-based therapies, several areas require further investigation to ensure their long-term safety in adolescents: Long-Term Metabolic Effects: Future studies should evaluate how sustained GLP-1RA use affects insulin sensitivity, lipid metabolism, and cardiovascular health over decades

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