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60 in a liquid scintillation spectrometer MicroBeta 2 Plate Counter (Perkin, USA)

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Chromatins were sonicated to 2001000 bp and immunoprecipitated with antibodies against NRF2, H3K4Me3, and H3K9Ac and determined with gene-specific primers (Supplementary Table 1)

Dosing considerations differ, and tesamorelin is contraindicated during pregnancy