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MT-1 is preferred when MC1R-specific data is required, while MT-2 is used in studies requiring broader melanocortin system activation

However, during high-fat diet (HFD) feeding, obesity and insulin resistance, the accumulation of medium-chain and long-chain acylcarnitines due to incomplete -oxidation may negatively regulate the expression of nuclear-encoded mitochondrial genes, further reducing mitochondrial number and function and creating a vicious metabolic cycle [33]