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This double-blind placebo-controlled study was performed between May 2011 and October 2016 at Nova Southeastern University (Fort Lauderdale, FL, USA) at three locations: The Mailman Segal Center (informed consent and behavioral assessments: visits 1, 3, 4, 5, 6, and 7), Nova Southeastern University Clinic (pediatric visits 2 and 8, including blood sampling), and the blood samples were quantified at the College of Pharmacy

The presence of benzyl alcohol ensures that the solution remains bacteriostatic after the first needle insertion occurs

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