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Fluorescent hemocyte reporter analysis Larvae were reared at 29 C until the 3rd instar stage was reached, washed 3 times with filtered water and individually dissected in 20 l of 1% Bovine Serum Albumin (BSA) in 1 PBS

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Furthermore, we used a commercial glycolysis antibody panel to detect Pfkp, Pyruvate kinase M1 and M2 (Pkm1, Pkm2), Hexokinase 1, Hexokinase 2 and Pyruvate dehydrogenase 1 subunit (Pdh) by immunoblot analysis