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The development of high-resolution multidimensional separation techniques such as high-pressure liquid chromatography coupled with a 16-channel coulometric multi-electrode array system (HPLCCMEAS), can lead to revolutionary changes in our understanding at the molecular level ( ANTIOXIDANT DEFENSE SYSTEM GLUTATHIONE REDOX COUPLING AND NITRIC OXIDE SIGNALING Free radicals are unstable atoms or molecules with odd (unpaired) electron(s) that can start a toxic chain reaction on important cellular components such as DNA, or the cell membrane