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DP cells will slowly overflow in DMEM/F-12 (Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12), 20% (v/v) fetal bovine serum (FBS) supplemented with 100 units/ml penicillin and 0.1 mg/ml streptomycin (Invitrogen, Carlsbad, CA, and Sigma, St

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Organic mineral sources, including selenium-methionine, zinc-amino acid chelates, and mineral-enriched yeasts, generally exhibit superior intestinal bioavailability compared with inorganic salts, thereby facilitating more efficient systemic transport and subsequent deposition into eggs (144)