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A bias factor ( ), which quantifies the relative engagement of one signalling state over another compared with the reference peptide, was calculated using the following equation: Where E max is the maximal effect, EC 50 is the half maximal concentration of the peptide, lig is the peptide being tested (P5 or GLP-1)

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Mechanical overloading induces GPX4-regulated chondrocyte ferroptosis in osteoarthritis via Piezo1 channel facilitated calcium influx