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All of the studies used both in vivo and some form of in vitro methods of data collection in order to observe different treatment effects, allowing for biomechanical, functional (use of AFI index and load to failure), macroscopic and microscopic histological analysis

For example, immunogenic impurities Des-Leu7_TPT, Des-His9_TPT, and Des-Leu11_TPT all have modifications to TCR-facing residues in the purported Treg epitope sequence (frame 5), which reduced the JanusMatrix human homology relative to the API (see in silico scores listed in Figure 9 )

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