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Briefly, INS-1E cells were maintained in RPMI 1640 medium (Gibco) supplemented with 5% (v/v) heat-inactivated fetal bovine serum, 2 mM l-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 100 U/mL penicillin/streptomycin and 50 M 2-mercaptoethanol

Stroke Cerebral stroke, which includes ischemic and hemorrhagic types, remains one of the leading causes of death and prolonged disability globally, frequently leading to permanent brain injury [134]

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Since B12 is not created by the human body, it can be supplemented via this method if your diet is insufficient