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The samples were centrifuged using TOMY Flexin Bench Top centrifuge at 350 rpm/5 minutes, aliquoted in micro centrifuge tubes, and then stored at 80C

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4 Application of probiotics in animal models The therapeutic efficacy of probiotics in UC is well supported by multiple animal models, which enable systematic assessment of barrier restoration, immune modulation, oxidative stress mitigation, and microbiota rebalancing under controlled conditions