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Bioorg, Med Chem Let

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After just 4 weeks of daily use, participants showed an increase in beneficial bacteria like Bifidobacterium and a decrease in Desulfovibrio, one of the main hydrogen sulfide-producing bacteria

On day 6, cells were counted, and 100 l cell suspension (in DMEM media supplemented with only 10% FBS but no antibiotics) was added to the 96-well plates (containing siRNA and transfection mix) at the appropriate seeding density (12k for DMSO and 15k for etoposide-treated cells)