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However, in this pathway, melatonin increases the concentration and activation of ERK, a molecule that regulates protein transcription in the cell nucleus, by acting on PKA and decreasing the formation of cAMP that induces negative regulation of the expression of mitogenic proteins and the antiapoptotic gene Bcl-2 [28,30]

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Stage 5: Mastering WH-Questions In a small group discussion, a child gained confidence in asking WH-questions such as Why cant I have that? Participating in these structured yet flexible interactions allowed them to practice and refine their use of more complex sentence structures