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Blood collection and analysis During the week before initiation of the experimental protocol and 12 weeks after the initiation, fasting blood samples were taken from the antecubital vein into BD Vacutainer tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA)

(45) demonstrated that eight sessions of OFA (650 nm, 10 mW) over four weeks compared to a 20 mW regimen, targeting GB34 and Xuanzhong acupoint (GB39) for three minutes per point, following intervention, cartilage degeneration and proteoglycan content were assessed via carmine staining

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