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In conclusion, there is a need for more targeted and specialized studies investigating specific signaling pathways involved in the GM-gut-brain axis, comparable to those conducted in mammalian models 5.2 Immune system Along with nutrient metabolism, and energy homeostasis, the GM has been generally recognized as a significant component of the intestinal mucosal immune system including regulation of innate and adaptive immunity ( Figure 1 )

Primary antibodies were incubated overnight at 4 C, followed by Alexa Fluor-conjugated secondary antibodies