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Three hours later, 6 cm dishes were placed on ice, washed with 3 mL of ice-cold PBS, and lysed with 0.6 mL of 1:1 mixture of PBS and 1% dodecyltrimethylammonium bromide (DTAB) in 0.2 M NaOH

PlexusDx supports this conversation by offering genetic context about peptide pathway predispositions, which may inform discussions about mechanism selection

National surveillance of emergency department visits for outpatient adverse drug events in children and adolescents

Then the pellets were stained for 20 min in 100 l assay buffer containing 5 g ml 1 propidium iodide (Sigma-Aldrich, P4170) and 3 l Annexin VFITC (BioLegend, 0640906)