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First, GST or GST-ECM1 and TwinStrep-LRP1 proteins were purified with GST beads (Sangon Biotech, China) and TwinStrep beads (Sangon Biotech, China), respectively, from IPTG-induced BL21 bacterial cells (Sangon Biotech, China)

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CAR-ap-enriched CAR19-T cells demonstrate comparable antitumor activity to those enriched using antibodies ( 2.4 Bispecific aptamer The development of a bi-specific aptamer (Bi-apt) platform for cancer therapeutics is formed by two monomeric aptamers, which can specifically bind to two kinds of targets simultaneously with high affinity ( 2.5 Aptamers in combination therapy Aptamers versatility allows for their integration into combination therapies, enhancing their therapeutic potential ( Aptamers can be used as targeting agents to deliver chemotherapeutic drugs, siRNAs, or nanocarriers directly to tumor cells, reducing off-target effects and systemic toxicity ( in vitro

Achim K, et al