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All collected samples were diluted to a protein concentration of 1 ng/l, and 5 l of each sample was loaded on a Tris-Glycine gel (Novex Tris-Glycine Mini Protein Gels, 1020%, 1.0 mm, WedgeWell) for Western blot analysis

When Wnt ligands bind to Frizzled receptors on the cell surface, they activate a protein called Dishevelled

This dysregulated Ca 2+ overload disrupts the structural integrity of the erythrocyte membraneincluding damage to cytoskeletal proteins and lipid peroxidationand triggers downstream apoptotic signaling cascades (Wang et al., 2022)

Pharmacological features and therapeutic implications of plumbagin in cancer and metabolic disorders: a narrative review