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The inclusion of Vitamin B12, a complex organometallic compound, provides a critical cofactor for enzymes like methionine synthase, directly linking it to the methionine cycle and folate metabolism, as well as fatty acid oxidation

In line with strong and stable interactions of His1-Ala2 of GLP-1 compared with transient interactions of His1-Gly2 in exendin-4, truncation of these two N-terminal residues reduces GLP-1 affinity by 100-300 fold, whereas for exendin-4, this has no effect, albeit for both peptides, these residues are required for GLP-1R-mediated cAMP production 2225