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Importantly, extracellular glutamate levels are tightly regulated, as their overabundance at synapses are inked to glutamate-induced excitotoxicity, a condition caused by elevated extracellular brain glutamate concentrations combined with excessive glutamatergic stimulation, resulting in surges in intracellular calcium levels and the initiation of neurotoxic signaling pathways, that are implicated in several neurological pathologies, including neurodegenerative disorders 6,8
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u.) was measured in the whole synaptophysin positive region
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