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, gravitational, centrifugal, etc.) in the presence or the absence of 3D matrices ( in vitro preclinical models to clinical applications ( In the context of in vitro models, a significant milestone is marked by the development of organ-on-chip (OoC) technology since they offer superior control over the cellular microenvironment, enable dynamic perfusion and mechanical stimulation, support real-time monitoring, and allow multi-tissue integration with higher reproducibility compared to organoids and spheroids ( OoC are microfluidic devices generally fabricated exploiting techniques common to microelectronics, such as photolithography ( in vivo ( Figure 1