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Briefly, 8-m-thick polycarbonate membranes were coated with 50 L 1.0% gelatin (Sigma-Aldrich) for 1 h

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The number of CD3+, CD4+, and CD8+ cells in spleen cells and draining lymph node cells was substantially decreased in tumour necrosis factor-induced protein-8 / mice, and CD4+ and CD8+ cells also showed a decreased capacity to produce interferon based on activation of the TANK-binding kinase 1 signalling axis and the upregulation of GPX4