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Measurement of the TNF- and IL-1 by ELISA The TNF- and IL-1 levels in the perihematomal were determined using ELISA kits (USCN Life Science Inc., Wuhan, China) according to the manufacturers instructions

Several lines of evidence suggest that metformin improves intestinal microflora composition and intestinal barrier dysfunction (4345), suggesting that this pathway might be essential for metformin against NALFD