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cAMP live imaging cAMP cADDis biosensor imaging INS-1 832/3 SNAP/FLAG-hGLP-1R WT versus V229A cells seeded onto glass bottom MatTek dishes, or pancreatic islets, were transduced overnight with the Green Up cADDis biosensor in a BacMam vector (#U0200G, Montana Molecular), according to the manufacturers instructions

Conversely, hepatocytes with acquired O-GlcNAc modification exhibited reduced necroptosis and liver fibrosis, indicating that OGT has the potential to inhibit necroptosis in hepatocytes and suppress the advancement of liver fibrosis

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