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For euthanasia, CO 2 (1.52 L/min) was pumped at a constant rate into sealed cages to sacrifice the rats

A secondary analysis from fecal samples collected from these volunteers identified Bacteroides ovatus and Phocaeicola dorei , which positively correlated with unconjugated BAs, and Bifidobacterium adolescentis, Collinsella aerofaciens , and Faecalibacterium prausnitzii , which positively correlated with secondary BAs, as the bacterial species candidates that affected gut BA metabolism (187)

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This is not to discourage research use, but to contextualise the evidence base accurately