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Several loading methods are available for modifying exosomes, including incubation, sonication, electroporation, transfection, extrusion, freeze-thaw cycles, thermal shock, saponin-assisted loading, pH gradient methods, and hypotonic dialysis

92 Endoplasmic reticulum stress treatment mainly improves the symptoms of UC by regulating endoplasmic reticulum function and reducing the abnormal accumulation of intracellular proteins, thereby inhibiting the inflammatory response of cells

Therefore, they require external antioxidant support in the form of oral glutathione supplements in Pakistan, like Gluvit-S Glutathione Oral Liquid

Critical unresolved issues include identifying the molecular switches that govern the transition of autophagy from an anti- to a pro-ferroptotic state, elucidating subtype-specific differences in crosstalk mechanisms, and clarifying the molecular basis underlying proteins such as COX7A1 to exhibit opposing functions