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cAMP/Gs and Gq- IP1assay To investigate the signaling downstream of GPR146, cellular cAMP level, which reflects the activation status of Gs or Gi signaling, was quantified using cAMP-Gs dynamic kit (PerkinElmer) following the manufactures protocol

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For its next stage in global domination, the format is going to foreign shores, with versions in Canada, Chile and Indonesia already under way and about to hit the airwaves in the UK (8 January on Channel 5), Philippines (February) and China (TBA)