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This effect is particularly pronounced in individuals who may already have a predisposition to gastrointestinal issues, making it essential for these patients to approach treatment with caution

Psychiatry , 2013 , 52 (3), 231-240

Glomerular endothelial cell senescence drives age-related kidney disease through PAI-1

By layering dissociated spinal cord cells over a four-step discontinuous gradient of 4.5%, 6%, 7.5% and 10.5% (w/v) iodixanol (OptiPrep initially diluted to 30% iodixanol with 0.15 M NaCl, 10 mM MOPS, pH 7.4 and then further diluted with Hanks Balanced Salt Solution) and centrifuging at 1900 rpm for 15 min., debris remained at the top interface, neurons banded at the three lower interfaces and inflammatory cells and glial cells pelleted [22,23]