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The respiration buffer contained 250 mM sucrose, 0.5 mM EDTA, 15 mM KCl, 20 mM KH2PO4, and 10 mM Tris-HCl

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In addition to inducing HF by promoting atherosclerotic progression, elevated plasma TMAO levels may accelerate myocardial hypertrophy, exacerbate mitochondrial dysfunction, and induce transverse-tubule (T-tubule) remodeling in cardiomyocytes (93)