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[9] [10] One standard drink contains approximately 14 grams of pure alcohol: 12 ounces of regular beer (5% alcohol), 5 ounces of wine (12% alcohol), or 1.5 ounces of distilled spirits (40% alcohol)
High-pH RP fractionation was performed at a flow rate of 0.6 mL/min with a gradient starting at 5% B for 2 min, increased to 50% B in 12 min, to 70% B within 0.5 min, and held for 6.5 min
Clear spirits and light beer beat dark spirits and red wine at equivalent ethanol doses not as a personal preference, but as a measurable chemistry difference
VSMCs were isolated from the dissected aorta using enzymatic dissociation solution [HEPES dissolved in HBSS (15 mM, pH: 7.2-7.3), BSA (2 mg/ml), CaCl2 (0.2 mM), Soybean Trypsin Inhibitor (0.25 mg/ml), elastase (0.0625 mg/ml) and collagenase (0.5 mg/ml)] (39)
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