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Aqueous solvents such as sterile water or bacteriostatic water are preferred for in vivo rodent studies because they eliminate the confounding variable of DMSO's own biological activity, which includes membrane permeabilization and anti-inflammatory effects

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Since the oral form of the peptide is not available commercially and injection of the peptide is not approved by the FDA, no metabolism experiments could be performed in humans If we go back to the 2014 review of six randomized, double-blind, placebo-controlled trials involving just over 900 patients, we see the following results: AOD9604 had no effect on serum IGF-1 levels, which confirms the hypothesis that AOD9604 does not act via IGF-1

However, its common for blood tests to reveal inaccurate information regarding B12 levels since a high percentage is stored in the liver and not always revealed through testing