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Eighty-nine C57BL/6 mice, 62 Sirt3 f/f mice (Wild type) and 75 Sirt3 f/f ;Cx3cr1-Cre mice (Sirt3 cKO) (purchased from Shanghai Model Organisms, China) were randomly assigned into different experimental groups according to different study designs (details were available in Tab S9)

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Nicotinamide Adenine Dinucleotide (NAD+) is a coenzyme found in every cell of your body, responsible for cellular energy production, DNA repair, and brain function