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Rights and permissions About this article Cite this article Wang, Y., Bikle, D

Following alkylation with 500 mM iodoacetamide and reduction with 500 mM DTT, pellets were digested overnight at 37C with 1 mg/mL Trypsin/Lys-C

For example, GluK2 and GluK5 are expressed in the majority of hippocampal neurons, as well as in various types of interneurons (Paternain et al., 2000), GluK1 exhibits widespread expression in cerebellar cortex and olfactory bulbs during early development (Pinheiro et al., 2007), and GluK4 is mainly present in the pyramidal neurons of the DG and Cornu Ammonis 3 (CA3) region of hippocampus and in the amygdala (Lerma and Marques, 2013