For the quantification of sulfur metabolites in serum, the metabolites in 20 uL of serum were extracted and derivatized by NEM in 80 L of ice-cold extraction solvent (80% MeOH: 20% H2O containing 25 mM NEM and 10 mM ammonium formate, pH = 7.0) which includes stable isotope labeled internal standards (1 M [ 13 C 3 , 15 N]-cysteine-NEM, 0.1 M [ 13 C 2 , 15 N]-GSH-NEM, 2 M [D 4 ]-Cystine, 4.6 M [ 13 C 5 , 15 N 2 ]-GSSG, 5 M [D 4 ]-Hypotaurine, and 40 M [ 13 C 2 ]-Taurine followed by incubation at 4 C for 30 min
Recommendation: However, the results may vary from person to person
In addition to the direct impact of high blood sugar, insulin resistance is also an essential factor leading to the dysfunction of BMSCs
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