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Sin embargo, si se detectan niveles de calcitonina marcadamente elevados, se justifica una evaluacin adicional

This is called extra-label or off-label use because this use isnt described on the drug label

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Cells were grown for 1 day, then transfected with 1 g GLP-1R construct (pcDNA6-GLP-1R-3xFLAG) using Lipofectamine 2000 (DNA/Lipofectamine 2000 ratio of 1:2) in each well